RPA1 anticorps (AA 350-400)

N° du produit ABIN7453556

L’anticorps Lapin Polyclonal anti-RPA1 a été validé pour WB, IP et IHC (fp). Il convient pour détecter RPA1 dans des échantillons de Humain et Souris.

Aperçu rapide pour RPA1 anticorps (AA 350-400) (ABIN7453556)

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPA1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Détail du produit anti-RPA1 anticorps

Épitope: AA 350-400

Fonction: Rabbit anti-RPA70 Antibody, Affinity Purified

Homologie: Orangutan

Purification: Affinity Purified

Immunogène: between AA 350 and 400

Isotype: IgG

Information d'application

Indications d'application:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Date de péremption: 12 months

Détails sur RPA1

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Autre désignation: RPA70

Sujet: Background: Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32 kDa (RPA2), and 14 kDa (RPA3). RPA1, the 70 kDa subunit, is an ssDNA binding protein that has been shown to be phosphorylated by ATM and ATR in response to DNA damage. In addition to a role in MMR, RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication.

ID gène: 6117

NCBI Accession: NP_002936

UniProt: P27694

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA